Part:BBa_K5406001:Design
Polyphosphatate kinase (PPK)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 311
Illegal PstI site found at 1708 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 311
Illegal PstI site found at 1708 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2052
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 311
Illegal PstI site found at 1708 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 311
Illegal PstI site found at 1708 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There were several design considerations: 1) Making sure the part was capable to be synthesized by Integrated DNA Technologies (IDT), 2) The part must be compatible with the Type IIS standard. That led to the removal of several restriction sites, 3) The part must contain the required codons so it was possible to express it in E.coli.
Source
Thermosynechococcus elongatus BP-1
References
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