Part:BBa_K5406000:Design
Bi-functional Glutathione Synthetase
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 763
Illegal PstI site found at 22
Illegal PstI site found at 52
Illegal PstI site found at 409
Illegal PstI site found at 1051
Illegal PstI site found at 1111 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 763
Illegal PstI site found at 22
Illegal PstI site found at 52
Illegal PstI site found at 409
Illegal PstI site found at 1051
Illegal PstI site found at 1111 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 763
Illegal BglII site found at 453 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 763
Illegal PstI site found at 22
Illegal PstI site found at 52
Illegal PstI site found at 409
Illegal PstI site found at 1051
Illegal PstI site found at 1111 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 763
Illegal PstI site found at 22
Illegal PstI site found at 52
Illegal PstI site found at 409
Illegal PstI site found at 1051
Illegal PstI site found at 1111
Illegal NgoMIV site found at 262
Illegal NgoMIV site found at 1497
Illegal NgoMIV site found at 1705 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There were several design considerations: 1) Making sure the part was capable to be synthesized by Integrated DNA Technologies (IDT), 2) The part must be compatible with the Type IIS standard. That led to the removal of several restriction sites, 3) The part must contain the required codons so it was possible to express it in E.coli.
Source
Streptococcus sanguinis SK36
References
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12. The PyMOL Molecular Graphics System, Version 4.6 Schrödinger, LLC.
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