Coding

Part:BBa_K5403997

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-10-01)


GFP as a C-terminal domain for M. smegmatis fusion proteins

This GFP was designed to be used as the C-terminal domain of M. smegmatis fusion proteins (BBa_K5403014-BBa_K5403020). Therefore, it does not contain a start codon and it was codon optimized for expression in M. smegmatis.

References

RCSB PDB (2005). PDB - 1W7S: Wild-Type Aequorea victoria Green Fluorescent Protein. https://www.rcsb.org/structure/1W7S

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 418
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None