Composite

Part:BBa_K5403018:Design

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-10-02)


Fusion protein for porinMspA-based membrane anchoring of GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 997
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 457
    Illegal AgeI site found at 511
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized for expression in M. smegmatis. Several modelling tools were used to aid in the design of this fusion protein (DeepTMHMM, Alphafold).



Source

M. smegmatis (PorinMspA) and Aequorea victoria (GFP)

References