Composite
Part:BBa_K5403018:Design
Designed by: Lizette van der Ziel Group: iGEM24_TU-Eindhoven (2024-10-02)
Fusion protein for porinMspA-based membrane anchoring of GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 997
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 457
Illegal AgeI site found at 511 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon-optimized for expression in M. smegmatis. Several modelling tools were used to aid in the design of this fusion protein (DeepTMHMM, Alphafold).
Source
M. smegmatis (PorinMspA) and Aequorea victoria (GFP)