Composite

Part:BBa_K5403016:Design

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-10-02)


Fusion protein for porinMspA-signal-sequence-based membrane anchoring of GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 592
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon-optimized for expression in M. smegmatis. Several modelling tools were used to aid in the design (DeepTMHMM, Signal6.0, Alphafold).


Source

M. smegmatis (PorinMspA signal sequence) and Aequorea victoria (GFP).

References