Coding

Part:BBa_K5403011:Design

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-10-02)


Membrane protein FtsQ of M. smegmatis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 41
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 41
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 41
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 41
    Illegal NgoMIV site found at 708
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 375
    Illegal BsaI.rc site found at 136
    Illegal SapI.rc site found at 203
    Illegal SapI.rc site found at 287


Design Notes

The sequence was codon-optimized for expression in M. smegmatis.


Source

M.smegmatis

References