Coding
Part:BBa_K5403011:Design
Designed by: Lizette van der Ziel Group: iGEM24_TU-Eindhoven (2024-10-02)
Membrane protein FtsQ of M. smegmatis
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 41
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 41
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 614
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 41
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 41
Illegal NgoMIV site found at 708
Illegal AgeI site found at 151 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 375
Illegal BsaI.rc site found at 136
Illegal SapI.rc site found at 203
Illegal SapI.rc site found at 287
Design Notes
The sequence was codon-optimized for expression in M. smegmatis.
Source
M.smegmatis