Part:BBa_K5403006

N-terminal signal sequence of OmpAtb

Outer membrane protein A (OmpA) is a protein found in E. coli that has been used as an anchor to attach other proteins to the membrane by recombinant expression of a fusion protein. OmpA has an analog in mycobacterium tuberculosis (M. tbc) called OmpAtb. iGEM Eindhoven 2024 aims to create a fusion protein for anchoring of a protein into the membrane of mycobacterial vesicles, and chose this protein as one of the candidates. However, this protein has a virulent function, so it is not allowed to use it in an BSL-1 laboratory. However, the 72 N-terminal amino acids, which form the signal sequence that anchors OmpAtb into the membrane, are safe to use in a BSL-1 laboratory. Therefore, this basic part was created as the basis for fusion proteins (BBa_K5403014) and (BBa_K5403015).

References

MyCoBrowser. (n.d.). https://mycobrowser.epfl.ch/genes/Rv0899

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 30
  Illegal AgeI site found at 169
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 16