Coding
Part:BBa_K5403005:Design
Designed by: Lizette van der Ziel Group: iGEM24_TU-Eindhoven (2024-10-02)
Spycatcher with unique Cysteine at N-terminus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 58
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 169
Design Notes
A cysteine residue was introduced at the N-terminus for maleimide coupling.
Source
S.pyogenes