Plasmid

Part:BBa_K5401002:Design

Designed by: Ong Jie Hao   Group: iGEM24_NTU-Singapore   (2024-10-01)


pBRR322_T7Promoter_eCFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2948
    Illegal suffix found in sequence at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2948
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 2954
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2948
    Illegal suffix found in sequence at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2948
    Illegal XbaI site found at 2963
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2188

Design Notes

The design of the plasmid contains a medium-copy number plasmid backbone (BBa_J428341), T7 Promoter (BBa_K3457003), RBS (BBa_J428032), eCFP(BBa_E0020) and T7Terminator (BBa_J428091).

Source

Molecular cloning using ligation; sequence of individual parts were derived from iGEM Registry.

References