RBS

Part:BBa_K5398004

Designed by: Jingya Zhao   Group: iGEM24_NAU-CHINA   (2024-09-05)


RBS (before 5' intron)

This part is composed of the ribosome binding sites with 15 bp. In our project, we added this part into the circular mRNA (cmRNA) (5' side) to express the gene of interest.

If you want to know more about it, please click the link below. https://parts.igem.org/Part:BBa_K5398006.

Reference

[1] LIU L, WANG P, ZHAO D, et al. Engineering Circularized mRNAs for the Production of Spider Silk Proteins[J]. Appl. Environ. Microbiol., 2022, 88(8): e00028-22.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None