Generator

Part:BBa_K539731:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-24)

kivd (alpha-ketoisovalerate decarboxylase) under TetR repressible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Kivd is under tetR promoter that is constitutive ON or repressed by tetR. It can be applied in your interested part.

Source

Kivd is cloned form Lactococcus lactis subsp, lactis KF147, by Zeng lab, NCTU-Formosa.

References