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Composite

Part:BBa_K539691:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6087
    Illegal AgeI site found at 2564
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2150
    Illegal BsaI site found at 4984
    Illegal BsaI site found at 5241
    Illegal BsaI.rc site found at 535
    Illegal BsaI.rc site found at 1129
    Illegal BsaI.rc site found at 2969


Design Notes

Built in Plac, two strong and one weak expressing RBS, Alss, ilvC, ilvD, 37’celcius regulator RBS, tetR and terminator (Part:BBa_K539651 with Part:BBa_K539653).

All of those sequences(Alss ,ilvC and ilvD) had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.

Source

Both of the ilvC and ilvD come from the Escherichia coli str. K-12 substr. MG1655. However,the Alss is from Bacillus subtilis.

And the part which repress Ptet when the temperature up to 37'Celius is designed by 2010 iGEM NCTU_Formosa.


References