Intermediate

Part:BBa_K539643:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

a RBS (B0034)+ilvC with a RBS (B0034)+ilvD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 610
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 196
    Illegal BsaI site found at 3027
    Illegal BsaI site found at 3284
    Illegal BsaI.rc site found at 1015


Design Notes

Built in two strong expressing RBSs, ilvC and ilvD (Part:BBa_K539623 with Part:BBa_K539626). Both of ilvC and ilvD had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.

Source

Both of the ilvC and ilvD come from the Escherichia coli str. K-12 substr. MG1655.

References