Generator

Part:BBa_K539631:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

promoter (lacI regulated) with a RBS (B0032)+Alss


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 535
    Illegal BsaI.rc site found at 1129


Design Notes

Built in Plac (Part:BBa_R0010), weak expressing RBS, and Alss (Part:BBa_K539627). The sequence of Alss had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.

Source

The Alss sequence comes from Bacillus subtilis.

References