Intermediate

Part:BBa_K539626:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

a RBS (B0034)+ilvD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1739
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1525
    Illegal BsaI site found at 1782


Design Notes

Built in strong expressing RBS (Part:BBa_B0034) and ilvD (Part:BBa_K539712).The sequence of ilvD had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.

Source

The sequence of ilvD comes from Escherichia coli str. K-12 substr. MG1655

References