Composite

Part:BBa_K539558:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

a RBS+lacI repressor+RNA thermometer+tetR+double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1930
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Built in strong expressing RBS(part:BBa_B0034), LacI(Part:BBa_C0012), 37’celcius regulator RBS, tetR and Terminator(Part:BBa_K332033).

Source

The device which can repress Ptet when the temperature up to 37'Celcius comes from 2010 iGEM NCTU_Formosa.

References