Coding

Part:BBa_K5394227

Designed by: Maria Isabella Perez   Group: iGEM24_IM-PANAMA   (2024-10-01)


cp157 Venus


Circularly permuted fluorescent proteins (cpFPs) have been used to develop biosensors that can monitor various intracellular events. When the N-terminus and C-terminus, are connected via a peptide linker new ends are created, close to the chromophore enhancing the mobility and spectral properties of cpFPs. GFP-like proteins have a rigid β-barrel structure with 11 β-sheets that house an internal distorted helix, resistant to conformational changes. Circular permutation involves joining the natural termini of a protein with a peptide linker and creating new termini elsewhere in the sequence so that they are less limited by their environment.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None