Coding
Part:BBa_K5394227
Designed by: Maria Isabella Perez Group: iGEM24_IM-PANAMA (2024-10-01)
cp157 Venus
Circularly permuted fluorescent proteins (cpFPs) have been used to develop biosensors that can monitor various intracellular events. When the N-terminus and C-terminus, are connected via a peptide linker new ends are created, close to the chromophore enhancing the mobility and spectral properties of cpFPs. GFP-like proteins have a rigid β-barrel structure with 11 β-sheets that house an internal distorted helix, resistant to conformational changes. Circular permutation involves joining the natural termini of a protein with a peptide linker and creating new termini elsewhere in the sequence so that they are less limited by their environment.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |