Protein_Domain

Part:BBa_K5394226

Designed by: Maria Isabella Perez   Group: iGEM24_IM-PANAMA   (2024-10-01)


medium flexible EL linker

This linker was made to optimize BRET in a chimeric LuxB: Venus protein. The linker should be of optimal length to maintain the donor and acceptor within the critical proximity range (<10 nm) necessary for BRET to occur. Typically, linkers of 10-15 amino acids are used, but this can vary depending on the specific proteins involved.

A flexible linker is utilized when the joined domains require a certain degree of movement or interaction. A medium linker was used to obtain a balence between stability and flexibility for ease of movement

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal BamHI site found at 37
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None