Protein_Domain

Part:BBa_K5394221:Design

Designed by: Maria Isabella Perez   Group: iGEM24_IM-PANAMA   (2024-10-01)


short flexible EL linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A BamHI restriction site was used to preserve the flexibility of the linker.


Source

An EL linker was used in " Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer" (Kaku, T., Sugiura, K., Entani, T. et al.) in order to construct the chimeric protein and validate of BRET for bacterial luciferase

References