Signalling

Part:BBa_K5384006:Design

Designed by: Si Cheng   Group: iGEM24_HBUT-Wuhan   (2024-08-24)


3x(6xHis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Histidine tag, also known as His-tag, is one of the simplest and most widely used purification tags with six or more consecutive histidine residues, which can be inserted into the C-terminus or N-terminus of the target protein.

Source

Currently, the purification of genetically engineered proteins with His-tag is mostly performed by immobilized metal chelate chromatography, which utilizes the interaction between the transition state metal ions and the histidine side chain to isolate the target protein.By literature:LIU Chengjun, HUANG Qing, ZHAO Lian, et al. Construction of Escherichia coli-Bacillus subtilis shuttle plasmid vector pSUGV4[J]. Journal of Sichuan University (Natural Science Edition),2001,38(2):243-246.] DOI:10.3969/j.issn.0490-6756.2001.02.024.

References

[1]WANG Wenli,WANG Yunlong,LI Chenyang,et al. Preparation, characterization and preliminary application of His-tagged monoclonal antibodies[J]. Journal of Cellular and Molecular Immunology,2008,24(4):399-400. DOI:10.3321/j.issn:1007-8738.2008.04.028. [2]HUA Wei-Wei,XU Wei-Yuan,HUANG Jian-Ying,et al. Synthesis and application of histidine-tagged protein purification media[J]. Chinese Journal of Food,2016,16(10):80-86. DOI:10.16429/j.1009-7848.2016.10.011.