Part:BBa_K5384003

Asp-Pro (acid sensitive site)

Asp-Pro acid-sensitive sites are regions of aspartic acid (Asp) and proline (Pro) in protein or peptide that are sensitive to acidic environments. Under acidic conditions, specific structural changes or chemical reactions may spontaneously occur at this site. The presence of such acid-sensitive sites may affect the overall structure and stability of the protein. It can be used to design acid-sensitive drug delivery systems. It remains stable in a normal physiological environment, but changes in a specific acidic pathological environment (such as tumor tissue, lysosomes, etc.), resulting in the release of the drug. In some biological processes, it may be involved in the folding, degradation, or functional regulation of proteins.

Usage and Biology

We introduce Asp-Pro acid-sensitive sites between NK and Vg, using acid to cleave the fusion protein. Acid sensitive site, which is distributed between Vg and Vg and other elements,resulting in separate Vg in acidic environment. Aspartic acid and glutamic acid are both acidic amino acids, and the pKa is about 3.9.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Application

In order to facilitate the separation of the target peptide from the fusion protein, the acid-sensitive aspartyl-proline (Asp-Pro) sites are added in the expression plasmid pPIC9K-3Vg-His.

Herein, we used the acid-sensitive site to connect NK ang Vg.