Coding

Part:BBa_K5382122

Designed by: yuyanyan chen   Group: iGEM24_HUBU-4-CHN   (2024-09-25)


HRV 3C site-enzyme cleavage site

The HRV 3C protease is an enzyme commonly used in the field of biotechnology, which is able to specifically recognize and cut a specific amino acid sequence Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro, thereby cutting the affinity label on the fusion protein. The cutting site of HRV 3C is here to facilitate the removal of His tags by immobilized metal affinity chromatography (IMAC) for protein affinity purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None