Part:BBa_K5382110

Inak-linker-z domain_protein domain that have the ability to bind to lgG

The InaK is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of Escherichia coli Nissle1917 cells as an anchor moda, so as to enable surface display.
The linker here is a short peptide used to connect the InaK and z domain.
The z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG)^[1]. In our project, the z domain is used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues

References

[1] Oscar P B Wiklander;Doste R Mamand;Dara K Mohammad;Wenyi Zheng;Rim Jawad Wiklander;Taras Sych;Antje M Zickler;Xiuming Liang;Heena Sharma;Andrea Lavado;Jeremy Bost;Samantha Roudi;Giulia Corso;Angus J Lennaárd;Manuchehr Abedi Valugerdi;Imre Mäger;Evren Alici;Erdinc Sezgin;Joel Z Nordin;Dhanu Gupta;André Görgens;Samir El Andaloussi.Antibody-displaying extracellular vesicles for targeted cancer therapy.[J].Nature biomedical engineering,2024,() Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1674
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 722
  Illegal NgoMIV site found at 962
  Illegal NgoMIV site found at 1130
  Illegal AgeI site found at 517
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 692
  Illegal BsaI.rc site found at 1182
  Illegal SapI.rc site found at 326