Part:BBa_K538130:Design

pBAD + RBS

K538130

This brick was used by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] to create CryoBricks; cold resistance BioBricks. It serves as a construction intermediate for one or several of their protein generators. Neither of this brick's subparts was designed by the team, though; the incorporated promoter (BBa_K20600, submitted by [http://2009.igem.org/Team:British_Columbia British Columbia 2009]) and RBS (BBa_B0034) were both taken from 2011's Spring Distribution Kit.

For more information about the subparts' purpose and design, refer to their main page or design page, respectively.

• BBa_K206000
  • Main Page
  • Design Page
• BBa_B0034
  • Main Page
  • Design Page

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]