Coding

Part:BBa_K5378006:Design

Designed by: pan zhao   Group: iGEM24_SMU-GDMU-CHINA   (2024-09-25)


tynA-G494S


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 267
    Illegal AgeI site found at 522
    Illegal AgeI site found at 2074
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TynA is a periplasmic amine oxidase containing copper and topaquinone cofactors, which converts amines to aldehydes.TynA-G494S discovered by Rottinghaus, A. G. et al converts phenethylamine (PEA) to phenylacetaldehyde (PAG) specifically.


Source

Escherichia coli

References

Reference:Rottinghaus, A. G., Xi, C., Amrofell, M. B., Yi, H., & Moon, T. S. (2022). Engineering ligand-specific biosensors for aromatic amino acids and neurochemicals. Cell systems, 13(3), 204-214.