Part:BBa_K5377300:Design
thiM TPP riboswitch
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
During the design process, attention should be paid to the compatibility of the fusion protein produced by the translation of the sequence following the riboswitch.
Source
It originates from the nuclear genome of Escherichia coli (E. coli).
References
[1]Rentmeister, A., Mayer, G., Kuhn, N., & Famulok, M. (2007). Conformational changes in the expression domain of the Escherichia coli thiM riboswitch. Nucleic acids research, 35(11), 3713–3722. https://doi.org/10.1093/nar/gkm300
[2]Sudarsan, N., Cohen-Chalamish, S., Nakamura, S., Emilsson, G. M., & Breaker, R. R. (2005). Thiamine pyrophosphate riboswitches are targets for the antimicrobial compound pyrithiamine. Chemistry & biology, 12(12), 1325–1335. https://doi.org/10.1016/j.chembiol.2005.10.007
[3]Thore, S., Leibundgut, M., & Ban, N. (2006). Structure of the eukaryotic thiamine pyrophosphate riboswitch with its regulatory ligand. Science (New York, N.Y.), 312(5777), 1208–1211. https://doi.org/10.1126/science.1128451