RNA

Part:BBa_K5377300:Design

Designed by: Jingyi Yang   Group: iGEM24_ZJUT-China   (2024-09-10)


thiM TPP riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the design process, attention should be paid to the compatibility of the fusion protein produced by the translation of the sequence following the riboswitch.


Source

It originates from the nuclear genome of Escherichia coli (E. coli).

References

[1]Rentmeister, A., Mayer, G., Kuhn, N., & Famulok, M. (2007). Conformational changes in the expression domain of the Escherichia coli thiM riboswitch. Nucleic acids research, 35(11), 3713–3722. https://doi.org/10.1093/nar/gkm300
[2]Sudarsan, N., Cohen-Chalamish, S., Nakamura, S., Emilsson, G. M., & Breaker, R. R. (2005). Thiamine pyrophosphate riboswitches are targets for the antimicrobial compound pyrithiamine. Chemistry & biology, 12(12), 1325–1335. https://doi.org/10.1016/j.chembiol.2005.10.007
[3]Thore, S., Leibundgut, M., & Ban, N. (2006). Structure of the eukaryotic thiamine pyrophosphate riboswitch with its regulatory ligand. Science (New York, N.Y.), 312(5777), 1208–1211. https://doi.org/10.1126/science.1128451