RNA

Part:BBa_K5377000:Design

Designed by: Jiyue Hu   Group: iGEM24_ZJUT-China   (2024-09-09)


ribB FMN riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the design process, attention should be paid to the compatibility of the fusion protein produced by the translation of the sequence following the riboswitch.


Source

It originates from the nuclear genome of Escherichia coli (E. coli).

References

[1]Meyer, A., Pellaux, R., Potot, S., Becker, K., Hohmann, H. P., Panke, S., & Held, M. (2015). Optimization of a whole-cell biocatalyst by employing genetically encoded product sensors inside nanolitre reactors. Nature chemistry, 7(8), 673–678. https://doi.org/10.1038/nchem.2301
[2]Serganov, A., Huang, L., & Patel, D. J. (2009). Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch. Nature, 458(7235), 233–237. https://doi.org/10.1038/nature07642
[3]Winkler, W. C., Cohen-Chalamish, S., & Breaker, R. R. (2002). An mRNA structure that controls gene expression by binding FMN. Proceedings of the National Academy of Sciences of the United States of America, 99(25), 15908-15913. https://doi.org/10.1073/pnas.212628899