Coding

Part:BBa_K5374024:Design

Designed by: Langning Xu   Group: iGEM24_TestDaily-I-Beijing   (2024-09-24)


FTD-EGFP (Fibrinogen-like Domain). A fibrinogen-like domain involved in collagen and ECM remodeling,


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 163
    Illegal PstI site found at 304
    Illegal PstI site found at 629
    Illegal PstI site found at 1096
    Illegal PstI site found at 1237
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1438
    Illegal PstI site found at 163
    Illegal PstI site found at 304
    Illegal PstI site found at 629
    Illegal PstI site found at 1096
    Illegal PstI site found at 1237
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 163
    Illegal PstI site found at 304
    Illegal PstI site found at 629
    Illegal PstI site found at 1096
    Illegal PstI site found at 1237
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 163
    Illegal PstI site found at 304
    Illegal PstI site found at 629
    Illegal PstI site found at 1096
    Illegal PstI site found at 1237
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2081


Design Notes

Sequence optimized for stable fusion with EGFP, retaining FTD’s collagen-binding function and ensuring reliable fluorescence for bioactivity studies.


Source

FTD domain from fibrinogen-related proteins, with EGFP from BBa_K1911005.