Plasmid

Part:BBa_K5371202

Designed by: Haoyang Yu   Group: iGEM24_iZJU-China   (2024-09-29)


GAL4WTV2

GAL4WTV2 is a plasmid featuring upstream homologous region for Sir2 integration, mCherry for fluorescent reporting, P2A for connection, GAL4 for signaling, and downstream homologous region for PRP11 integration. It integrates a signaling and reporting section into genome of Saccharomyces cerevisiae to report expression of GAL4, while regulating downstream genes through GAL/UAS system. It was applied into our project to report the expression level of the SIR2 located upstream, while regulating the expression of ERG20-MrBBS through GAL/UAS system.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 4329
    Illegal XbaI site found at 1646
    Illegal PstI site found at 527
    Illegal PstI site found at 2289
    Illegal PstI site found at 4943
    Illegal PstI site found at 8417
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 4329
    Illegal PstI site found at 527
    Illegal PstI site found at 2289
    Illegal PstI site found at 4943
    Illegal PstI site found at 8417
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 4329
    Illegal BamHI site found at 48
    Illegal BamHI site found at 4060
    Illegal XhoI site found at 1158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 4329
    Illegal XbaI site found at 1646
    Illegal PstI site found at 527
    Illegal PstI site found at 2289
    Illegal PstI site found at 4943
    Illegal PstI site found at 8417
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 4329
    Illegal XbaI site found at 1646
    Illegal PstI site found at 527
    Illegal PstI site found at 2289
    Illegal PstI site found at 4943
    Illegal PstI site found at 8417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1077
    Illegal BsaI.rc site found at 2753
    Illegal BsaI.rc site found at 3942
    Illegal BsaI.rc site found at 6668
    Illegal BsaI.rc site found at 7727
    Illegal SapI site found at 1442
    Illegal SapI site found at 5585
    Illegal SapI site found at 7879


[edit]
Categories
Parameters
None