Part:BBa_K5371010
pHXT1
The HXT1 promoter is a regulatory DNA sequence found in the yeast Saccharomyces cerevisiae. It controls the expression of the HXT1 gene, which encodes a low-affinity glucose transporter. This transporter is part of the major facilitator superfamily and plays a crucial role in glucose uptake when glucose is abundant. The HXT1 promoter’s primary function is to regulate the expression of the HXT1 gene in accordance with glucose availability. This regulation is crucial for the efficient uptake of glucose when it is abundant, thereby supporting cellular metabolism and energy production. The HXT1 gene product, a glucose transporter, facilitates the translocation of glucose across the plasma membrane, which is essential for cellular metabolic processes. The HXT1 promoter can be employed to drive the expression of heterologous genes in yeast. By linking a gene of interest to the HXT1 promoter, researchers can investigate the gene’s function and regulation in response to glucose levels.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 120
Illegal SpeI site found at 779
Illegal PstI site found at 423 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 120
Illegal SpeI site found at 779
Illegal PstI site found at 423 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 120
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 120
Illegal SpeI site found at 779
Illegal PstI site found at 423 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 120
Illegal SpeI site found at 779
Illegal PstI site found at 423 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 727
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