Signalling

Part:BBa_K5371005:Design

Designed by: Haoyang Yu   Group: iGEM24_iZJU-China   (2024-09-28)


SIR2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 823
    Illegal BamHI site found at 261
    Illegal BamHI site found at 809
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the integrated oscillation system, SIR2 is regulated by CYC1 promoter. It is activated in case of HAP4 expression, which is one of the critical parts of the longevity oscillation that we constructed in yeast.


Source

The SIR2 was amplified from genome of Saccharomyces cerevisiae.

References