Protein_Domain

Part:BBa_K5371002:Design

Designed by: Yue Cao   Group: iGEM24_iZJU-China   (2024-09-25)


P2A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing the P2A sequence, high self-cleavage efficiency was ensured, and potential negative impacts on downstream protein functionality were minimized. The sequence was optimized to improve cleavage efficiency in various expression systems.


Source

The P2A peptide sequence is derived from the genomic sequence of the porcine teschovirus-1.

References