Coding

Part:BBa_K5371001:Design

Designed by: Yue Cao   Group: iGEM24_iZJU-China   (2024-09-25)


TSC10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 64
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 64
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing the TSC10 sequence, its role in the sphingolipid biosynthesis pathway was considered to ensure effective expression and functionality in heterologous expression systems. The sequence was optimized to enhance expression efficiency and avoid potential secondary structures.


Source

The TSC10 gene is derived from the genomic sequence of yeast (Saccharomyces cerevisiae).

References