Composite

Part:BBa_K537016:Design

Designed by: Ezio Fok   Group: iGEM11_WITS-CSIR_SA   (2011-09-16)

Promoter-RBS-CheZ-mCherry-Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1245
    Illegal AgeI site found at 1357
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coming soon


Source

Strong promoter – Bba_J23119

RBS CheZ N-Fusion - BBa_K537007

mCherry (mRFP1) C-Fusion - BBa_K537005

Double terminator – Bba_B0015

References