Composite

Part:BBa_K537010:Design

Designed by: Ezio Fok   Group: iGEM11_WITS-CSIR_SA   (2011-09-16)

Promoter-Theophylline riboswitch 2-Venus-Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coming soon


Source

Theophylline Riboswitch (type2) venus - BBa_K537004

Strong promoter – Bba_J23119

Double terminator – Bba_B0015

References

Lynch S.A. and Gallivan J.P., A flow cytometry based screen for synthetic riboswitches. 2009, Nucleic Acids Res. 37(1)184-192