Part:BBa_K537004:Design
Theophylline Riboswitch 2-Venus
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 687
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This biobrick part represents a composite of a theophylline riboswitch (type2/clone 12.1) upstream of a Venus fluorescent protein reporter (derived from part BBa_K354002).
Previous teams: Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010 all considered strategies for making the riboswitch a separate biobrick, however standard assembly techniques are inadequate since the sequence distance between the ATG, RBS and aptamer domain of the riboswitch are critical. We have explored this further in a more elaborate discussion here.
Source
The Venus fluorescent protein is derived from BBa_K354002.
References
1. Lynch S.A. and Gallivan J.P., A flow cytometry based screen for synthetic riboswitches. 2009, Nucleic Acids Res. 37(1)184-192
2. Topp S, Gallivan JP. Guiding bacteria with small molecules and RNA. J Am Chem Soc 2007;129:6807-11