Part:BBa_K5368001:Design
pU57-Tyr-anti-CD3 scfv-CD86-mut IL-2
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon Optimization: Ensure that the sequence uses codons preferred by the host organism (e.g., E. coli, mammalian cells) to enhance expression levels.
Functional Domains: Identify and retain critical functional domains of the protein to ensure proper folding and functionality.
Post-Translational Modifications: Consider potential post-translational modification sites (e.g., glycosylation, phosphorylation) that may be important for the protein's function.
Promoter Selection: Choose an appropriate promoter that drives effective expression in the intended host system.
Multiple Cloning Site (MCS): If using a vector, ensure the MCS contains suitable restriction sites for the insertion of the desired sequence.
Source
pU57:Extraction from Escherichia coli Tyr：The mouse tyrosinase promoter (Tyr promoter) is derived from the genomic sequence of the mouse tyrosinase gene, which is located on chromosome 7. CD3, CD86：Originates from the mouse genome. mut IL-2:We design mouse originated mimics of the central immune cytokine interleukin-2 (IL-2) that bind to the IL-2 receptor