Part:BBa_K5366051
pCDFDuet-P1-sfGFP1
Plasmids expressing green fluorescence
After several attempts, we selected M9 medium as a substitute; however, subsequent experiments yielded unsatisfactory results. Concerned that this might be the reason for the strong deterrent capability, we decided to truncate the gene cluster to diminish its deterrent effect. The S18700 fragment (1218 bp) was excised using the recombinant plasmid pCDFDuet-1-tagR-RS-GFP as the template, with P-tagR-For and pcdfduet-P1-tagR-sfGFP-REV primers. Colony PCR (3349 bp) was performed on the transformed colonies. Positive colonies were selected for plasmid extraction. Following sequencing verification, the recombinant plasmid pCDFDuet-P1-sfGFP1 was successfully obtained.
Fig. 1 Construction maps of plasmids of pcdfduet-tagrp1-sfgfp1
Fig. 2 Construction maps of plasmids of pcdfduet-p1-sfgfp1
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1056
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1056
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1056
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1056
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1056
Illegal AgeI site found at 417
Illegal AgeI site found at 540 - 1000COMPATIBLE WITH RFC[1000]
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