Regulatory

Part:BBa_K5366048

Designed by: Lin Li   Group: iGEM24_NJTECH-CHINA-A   (2024-09-27)


Feature 14

Bidirectional promoter.Feature 14 is a DNA sequence located upstream of the 5' end of a structural gene, facilitating the precise binding of RNA polymerase to the template DNA and ensuring specificity in transcription initiation. This sequence is situated between two adjacent genes that are transcribed in opposite directions and is classified as part of the tagatose-specific promoter. The general structure of the promoter comprises the core promoter element and the upstream regulatory element. Promoters play a crucial role in determining when and where a gene is expressed. Notably, this promoter enables recombinant plasmids to specifically recognize tagatose. In the presence of tagatose, it regulates gene expression and cell specificity, allowing for responses to environmental changes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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