Coding

Part:BBa_K535008:Design

Designed by: iGEM11_UNAM-Genomics_ Mexico   Group: iGEM11_UNAM-Genomics_Mexico   (2011-09-25)

repC - ctRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 835
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 916
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 761
    Illegal SapI site found at 955


Design Notes

In order to have the same result as in pUX19-XKR5 [1] there are 290 bps upstream from start transcription site of repC. To get some repC downstream sequence there are added 51bp.

Source

Low copy number plasmid pRmeGR4a from Sinorizobium meliloti GR4.

References

1. Izquierdo, Javier et al. An antisense RNA plays a central role in the replication control of a repC plasmid. 2005. Plasmid 54, 259-277.

2. Rogottier-Gois, Lionel. Distribution of repC plasmid-replication sequences among plasmids an sisolatees of Rhizobium leguminosarum bv. viciae from field populations. 1998. Microbiology, 144, 771-780.