Coding

Part:BBa_K5348029

Designed by: ER DU   Group: iGEM24_Songshan-Lake   (2024-09-23)


LEVI

Fusion component that the light-oxygen-voltage (LOV) domain-containing protein from Neurospora crassa fused to the C-terminus of DNA-binding domain of LexA repressor of the Escherichia coli SOS regulon. Upon blue light irradiation,a cysteine-flavin adduct is formed in the VVD domain, causing conformational changes of the domain and the subsequent dimerization of the fusion protein.The activated protein dimer would then bind its cognate operator sequence and repress the promoter activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 677
  • 1000
    COMPATIBLE WITH RFC[1000]


LEVI (BBa_K5348029)

LEVI (BBa_K5348029)

Profile

Name LEVI
Base Pairs 717 bp
Origin Synthesized
Properties LEVI is a fusion component that the light-oxygen-voltage (LOV) domain-containing protein from Neurospora crassa fused to the C-terminus of the DNA-binding domain of LexA repressor of the Escherichia coli SOS regulon. Upon blue light irradiation, a cysteine-flavin adduct is formed in the VVD domain, causing conformational changes in the domain and the subsequent dimerization of the fusion protein. The activated protein dimer would then bind its cognate operator sequence and repress the promoter activity [1].
Figure 1. Schematic diagram of the role of LEVI
Figure 1. Schematic diagram of the role of LEVI [1].

Reference

[1] Chen, X., Liu, R., Ma, Z. et al. An extraordinary stringent and sensitive light-switchable gene expression system for bacterial cells. Cell Res. 2016, 26, 854–857.

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