Plasmid

Part:BBa_K5338005

Designed by: David He   Group: iGEM24_Westview-SanDiego   (2024-10-02)

FastPETase L1 (Full Transcriptional Unit) Plasmid

This is the full Level 1 Plasmid, which contains the L1 PETase Transcriptional Unit in a pCA backbone. SAP1 sites are located in backbone for Loop Assembly. This plasmid can be directly transformed into E.Coli, or possibly combined with MHETase in a Golden-Gate Reaction to create a L2 plasmid with the PETase-MHETase pathway. This reaction was attempted but did not succeed for Westview iGEM.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2181
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2101
    Illegal NheI site found at 2124
    Illegal PstI site found at 2181
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2181
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2080
    Illegal SapI.rc site found at 1


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Categories
Parameters
None