Plasmid
Part:BBa_K5338005
Designed by: David He Group: iGEM24_Westview-SanDiego (2024-10-02)
FastPETase L1 (Full Transcriptional Unit) Plasmid
This is the full Level 1 Plasmid, which contains the L1 PETase Transcriptional Unit in a pCA backbone. SAP1 sites are located in backbone for Loop Assembly. This plasmid can be directly transformed into E.Coli, or possibly combined with MHETase in a Golden-Gate Reaction to create a L2 plasmid with the PETase-MHETase pathway. This reaction was attempted but did not succeed for Westview iGEM.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2181
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2101
Illegal NheI site found at 2124
Illegal PstI site found at 2181 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2181
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2181
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2080
Illegal SapI.rc site found at 1
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Categories
Parameters
None |