Regulatory

Part:BBa_K5338001

Designed by: My-Nga Ingram   Group: iGEM24_Westview-SanDiego   (2024-10-02)


Rhamnose Inducible Promoter

This is the a sequence for a rhamnose inducible promoter that was used in our attempted L2 FastPETase + MHETase plasmid. Genes of interest will be suppressed in the presence of glucose, but expression will occur with rhamnose. This rhamnose inducible promoter was used with the L2 plasmid to reduce MHETase production stress on cells, but L2s failed nevertheless. We also used it with RFP to demonstrate fluorescence/expression of our plasmid, but this was not successfully implemented (Gel Electrophoresis revealed that reactions failed).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 103
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 103
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 103
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 103
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 103
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None