DNA

Part:BBa_K5338000

Designed by: My-Nga Ingram   Group: iGEM24_Westview-SanDiego   (2024-09-30)


FastPETase

PETase is a protein isolated from Ideonella Sakeinsis, that is found to degrade PET plastics into PET and MHET monomers. FastPETase is a synthetically modified version of PETase that was designed to be more efficient and heat-tolerant. This gene was successfully implemented in Level 0 and Level 1 plasmid construct, and transformed into E.Coli. Current attempts are to create a Level 2 plasmid in conjunction with MHETase, and transforming into Alteromonas macleodii.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 26
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None