Coding

Part:BBa_K5335031

Designed by: Wentao Yang   Group: iGEM24_HZAU-China   (2024-10-02)


A tran-saconite transporter derived from Bacillus thuringiensis

A tran-saconite transporter derived from Bacillus thuringiensis,which could transport TAA from the inside to outside cell body.

Usage and Biology

TbrB is a transaconite transporter derived from Bacillus thuringiensis, which has the ability to transport TAA from the interior of the cell to the exterior. In Bacillus thuringiensis, it is located within the same gene cluster as TbrA. In our project, TAA serves as a nematode-killing small organic molecule. Therefore, TbrB is crucial for enhancing the nematode-killing efficacy of the engineered bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 639
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 639
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 639
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 639
    Illegal AgeI site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None