Composite

Part:BBa_K5335023:Design

Designed by: Ruichen Shen   Group: iGEM24_HZAU-China   (2024-09-28)


A part (including a reporter gene) that induces and regulates downstream gene expression by salicyli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1855
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that activation of Psal by NahR is dependent on the NahR binding sequence, which may not be annotated in some of the original documents.


Source

NahR, NahR binding sequence, and Psal from the naphthalene operon of Pseudomonas putida.

References