Coding

Part:BBa_K5335019:Design

Designed by: Wentao Yang   Group: iGEM24_HZAU-China   (2024-09-28)


The plant immune enhancers composed of VDAL, Nlp20 and Bacillus splip


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The splip must be placed in the 5'-terminal site, or it will be useless.


Source

artificial synthesis

References

[1]Böhm H, Albert I, Oome S, Raaymakers TM, Van den Ackerveken G, Nürnberger T. A conserved peptide pattern from a widespread microbial virulence factor triggers pattern-induced immunity in Arabidopsis. PLoS Pathog. 2014 Nov 6;10(11):e1004491. doi: 10.1371/journal.ppat.1004491. [2]Bian C, Cheng J, Han Y, Yang S, Gong Z, Qi J. Verticillium dahliae effector VDAL protects MYB6 from degradation by interacting with PUB25 and PUB26 E3 ligases to enhance Verticillium wilt resistance. Plant Cell. 2021 Dec 3;33(12):3675-3699. doi: 10.1093/plcell/koab221. [3]Jiang S, Zheng W, Li Z, Tan J, Wu M, Li X, Hong SB, Deng J, Zhu Z, Zang Y. Enhanced Resistance to Sclerotinia sclerotiorum in Brassica rapa by Activating Host Immunity through Exogenous Verticillium dahliae Aspf2-like Protein (VDAL) Treatment. Int J Mol Sci. 2022 Nov 12;23(22):13958. doi: 10.3390/ijms232213958. [4]14]Ma RJ, Wang YH, Liu L, Bai LL, Ban R. Production enhancement of the extracellular lipase LipA in Bacillus subtilis: Effects of expression system and Sec pathway components. Protein Expr Purif. 2018 Feb;142:81-87. doi: 10.1016/j.pep.2017.09.011. Epub 2017 Sep 27.