Coding

Part:BBa_K5335009:Design

Designed by: Lize Chen   Group: iGEM24_HZAU-China   (2024-09-28)


Bacillus subtilis optimized KillerRed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 506
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 151
    Illegal BsaI.rc site found at 413


Design Notes

We replace codons in the original mRNA sequence with codons that are used frequently in Bacillus subtilis to ensure that the codons in the newly designed mRNA sequence are compatible with the codon usage bias of Bacillus subtilis.


Source

KillerRed is engineered from anm2CP to be phototoxic.This part has been optimized in Bacillus subtilis.

References