Coding

Part:BBa_K5335009

Designed by: Lize Chen   Group: iGEM24_HZAU-China   (2024-09-28)


Bacillus subtilis optimized KillerRed

This part derivers from BBa_K1184000. We replace codons in the original mRNA sequence with codons that are used frequently in Bacillus subtilis to ensure that the codons in the newly designed mRNA sequence are compatible with the codon usage bias of our chassis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 506
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 151
    Illegal BsaI.rc site found at 413


Usage and Biology

Along with Pxyl (BBa_K143014), it was cloned into plasmid pHT315 and converted into Bacillus subtilis 168 for further detection. We can see phenotype directly from the color of the liquid.

Figure. Phenotypic result comparison chart.

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Parameters
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