Coding
Part:BBa_K5335009
Designed by: Lize Chen Group: iGEM24_HZAU-China (2024-09-28)
Bacillus subtilis optimized KillerRed
This part derivers from BBa_K1184000. We replace codons in the original mRNA sequence with codons that are used frequently in Bacillus subtilis to ensure that the codons in the newly designed mRNA sequence are compatible with the codon usage bias of our chassis.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 506
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 506
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 506
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 506
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 151
Illegal BsaI.rc site found at 413
Usage and Biology
Along with Pxyl (BBa_K143014), it was cloned into plasmid pHT315 and converted into Bacillus subtilis 168 for further detection. We can see phenotype directly from the color of the liquid.
Figure. Phenotypic result comparison chart.
[edit]
Categories
Parameters
None |