Coding

Part:BBa_K5335005

Designed by: Haozhen Liu   Group: iGEM24_HZAU-China   (2024-09-28)


It is a fusion protein composed of SpyCatcher, Cry6Aa2, (GGGS)4 and CPPs

Cry6Aa2 connects to Spycatcher and CPPs via Flexible linker(GGGS)4. CPPs can help Cry6Aa2 enter nematode cells to play a role, and Spycatcher can bind with Spytag through isopeptide bonds to load fusion proteins onto VLP (virus-like particles).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1456
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1456
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1561
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1456
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1456
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None